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Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice
《化学科学与工程前沿(英文)》 2023年 第17卷 第3期 页码 298-306 doi: 10.1007/s11705-022-2212-9
《化学科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 103-111 doi: 10.1007/s11705-021-2064-8
关键词: UDP-glucuronosyltransferase 1A8 fluorescent probe subtype selectivity fluorescence imaging
SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article
Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen
《工程(英文)》 doi: 10.1016/j.eng.2023.05.010
Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.
关键词: In vivo Short-wave infrared Skeletal muscle Macrophage Regeneration
荧光显纳镜在神经科学中的应用 Review
王杨云逗, 林剑, 张启明, 陈希, 栾海涛, 顾敏
《工程(英文)》 2022年 第16卷 第9期 页码 29-38 doi: 10.1016/j.eng.2020.11.010
荧光显纳技术提供了克服光学显微镜中衍射极限分辨率障碍的成像技术,从而开辟了生物医学成像研究领域(尤其在神经科学领域)。本文回顾了最重要的荧光显纳技术,包括描述了该技术在阐明蛋白质结构和流动性、神经活动过程中涉及突触参数的实时测定、三维成像和纳米级神经活动跟踪等方面的应用。最后,讨论了荧光显纳镜的前景,特别关注该技术在神经科学中与相关技术(如机器学习)的结合。
evaluation of renal function using diffusion weighted imaging and diffusion tensor imaging in type 2
null
《医学前沿(英文)》 2014年 第8卷 第4期 页码 471-476 doi: 10.1007/s11684-014-0365-8
This work aims to estimate the value of diffusion weighted imaging (DWI) and diffusion tensor imaging (DTI) in detecting early-stage kidney injury in type 2 diabetic patients with normoalbuminuria (NAU) versus microalbuminuria (MAU) prospectively. A total of 30 T2DM patients with normal kidney function were recruited and assigned to the NAU group (n = 14) or MAU group (n= 16) according to 8 h overnight urinary albuminuria excretion rate (AER) results. A contemporary cohort of health check-up recipients were included as controls (n = 12). DWI and DTI scans were performed on bilateral kidney using SE single-shot EPI, and apparent diffusion coefficient (ADC) and fractional anisotropy (FA) of the renal parenchyma was determined from ADC and FA maps of the three groups. ADC and FA values were compared among the three groups. According to DWI with a b value of 400 s/mm2, the MAU and NAU groups showed significantly lowered mean ADC values compared with the healthy controls (P<0.01). The mean ADC in the MAU group [(2.22±0.07)×10–3 mm2/s] was slightly lower than that of the NAU group [(2.31±0.22)×10–3 mm2/s], but this difference was not statistically significant (P>0.05). The FA value in the MAU group was higher than that in the control group (0.45±0.07 vs. 0.39±0.03, P = 0.004) but did not differ from that in the NAU group (0.42±0.03) (P>0.05). ADC and FA values may be more sensitive than urine AER in reflecting early-stage kidney injury and, hence, may facilitate earlier detection and quantitative evaluation of kidney injury in T2DM patients. Combined evaluation of ADC and FA values may provide a better quantitative approach for identifying diabetic nephropathy at early disease stages.
关键词: type 2 diabetes mellitus microalbuminuria diffusion weighted imaging diffusion tensor imaging early-stage kidney injury
基于临床可用的亚甲基蓝近红外二区成像用于泌尿系统结构及功能成像的研究 Article
薛丁玮, 吴迪, 陆泽毅, Jochen Neuhaus, 阿卜杜热合曼·则比布拉, 冯哲, 成晟, 周静, 钱骏, 李恭会
《工程(英文)》 2023年 第22卷 第3期 页码 149-158 doi: 10.1016/j.eng.2021.07.032
精准的结构和功能成像对于泌尿系统疾病的诊断和预后至关重要。近红外二区光谱区域(second nearinfrared spectral region, NIR-II, 1000~1700 nm)荧光生物成像相较于传统的荧光生物成像具有更高的空间分辨率、更深的穿透力和更好的信噪比,但其临床适用性有限。在此,本文首次报道了基于临床可用且经肾脏排泄的荧光染料亚甲基蓝(methylene blue, MB)对活体泌尿系统进行NIR-II 荧光成像,该技术不仅可实现清晰的有创/无创尿路造影,而且还能有效且无创地检测肾功能。以上研究结果表明MB辅助的NIR-II 荧光成像在临床/基础研究中泌尿系统结构和功能成像领域中具有较大的应用前景。
Xin ZHANG, Zichen LI
《化学科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 381-387 doi: 10.1007/s11705-013-1359-9
荧光碳点的快速宏量制备及多功能应用 Article
王丹,王志勇,詹求强,蒲源,王洁欣,Neil R. Foster,戴黎明
《工程(英文)》 2017年 第3卷 第3期 页码 402-408 doi: 10.1016/J.ENG.2017.03.014
荧光纳米材料在化学传感、生物成像及光电器件等领域具有重要应用前景,其合成方法的研究得到广泛关注。我们采用柠檬酸和乙二胺作为原料,在150 ℃、常压的相对温和条件下,通过“一锅法”反应制备了氮掺杂荧光碳点。所得荧光碳点光学带隙为3.4 eV,在单光子或双光子激发下呈现出明亮的荧光,且荧光发射波长稳定(λ = 450 nm)。基于荧光碳点低细胞毒性和长荧光寿命的优势,实现了荧光碳点作为离体肿瘤细胞(HeLa 细胞) 标记和荧光寿命成像探针的应用,获得了高对比度的荧光寿命成像结果。通过将所得荧光碳点与普通油墨混合制备均匀分散的荧光墨水,可用于普通纸张及其他基材( 如柔性塑料薄膜、纺织品、衣服) 的荧光图案化,为固态荧光传感、荧光防伪标记和可穿戴光电子设备应用研究提供了基础。
4Pi荧光超分辨显微术综述 Review
郝翔, 李依明, 傅爽, 李旸晖, 许迎科, 匡翠方, 刘旭
《工程(英文)》 2022年 第11卷 第4期 页码 146-153 doi: 10.1016/j.eng.2020.07.028
自20 世纪90 年代以来,持续的科技进步突破了光学显微镜的衍射极限,使三维超分辨显微成像技术得以实现。回顾这些历程,一个重要的里程碑是基于两个对置物镜的4Pi 显微架构及其超分辨版本的出现。鉴于此,本文综述了4Pi 超分辨显微术的近期进展。总体上,4Pi 超分辨显微镜为透明样品观测提供了一
种能够突破衍射极限、非侵入、各向同性的三维分辨率的技术手段。具体而言,本文针对目标开关和随机开关两个主要4Pi 超分辨显微术版本,讨论了它们的架构、原理、应用和未来发展趋势。
ZHU Xiaoqin, QIAN Ying, LU Zhifeng
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 381-384 doi: 10.1007/s11705-007-0069-6
关键词: 2-arylvinyl trans-vinylene character effective fluorescence push-core-pull-core-push
《化学科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 92-102 doi: 10.1007/s11705-021-2063-9
关键词: hydrolysis reaction mitochondrial DNA damage in situ hydrophobic environment trigger fluorescence probe apoptosis
In vivo imaging of hematopoietic stem cell development in the zebrafish
Panpan Zhang, Feng Liu
《医学前沿(英文)》 2011年 第5卷 第3期 页码 239-247 doi: 10.1007/s11684-011-0123-0
关键词:
hematopoietic stem cell
hematopoiesis
Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin
《环境科学与工程前沿(英文)》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1219-z
关键词: Fluorescence Photophysical properties Effect of the substituent Toxic aromatic compounds
Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1057-4
Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium than chromium. Indigenous Cd-tolerant EAB cytoplasm located more chromium than cadmium. Simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB was achieved. Current accelerated the harboring of cadmium in EAB at an initial 2 h. Current directed the accumulation of more chromium in EAB over time.
关键词: Microbial electrolysis cell Electrochemically active bacteria Cd-tolerant bacteria Cd(II) and Cr(VI) Fluorescence probe
LIU Feng, XIAO Jianhua, REN Weihua, HU Jun, LIU Honglai
《化学科学与工程前沿(英文)》 2008年 第2卷 第4期 页码 428-433 doi: 10.1007/s11705-008-0083-3
关键词: detection different fluorescence UV-Vis end-capped polymer
标题 作者 时间 类型 操作
A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living
期刊论文
SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal
Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen
期刊论文
evaluation of renal function using diffusion weighted imaging and diffusion tensor imaging in type 2
null
期刊论文
基于临床可用的亚甲基蓝近红外二区成像用于泌尿系统结构及功能成像的研究
薛丁玮, 吴迪, 陆泽毅, Jochen Neuhaus, 阿卜杜热合曼·则比布拉, 冯哲, 成晟, 周静, 钱骏, 李恭会
期刊论文
Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides
Xin ZHANG, Zichen LI
期刊论文
Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence
ZHU Xiaoqin, QIAN Ying, LU Zhifeng
期刊论文
hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA
期刊论文
Insight into fluorescence properties of 14 selected toxic single-ring aromatic compounds in water: Experimental
Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin
期刊论文
Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based on the sensing of fluorescence
Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li
期刊论文